Laboratory Animal Research Department

Humanized model laboratory

Currently, rodent models are the gold standard in most fields of biomedical researches due to their small size, large litters, and ease of maintenance, handling, and reproductive engineering. However, murine models cannot fully recapitulate the physiological and pathological mechanisms underlying most human diseases because the expression level or functional status of the molecules frequently differ between human and rodent cells. Therefore, humanized mouse models in which human cells or tissues are engrafted in immunodeficient mice are promising tools to overcome this issue.

In humanized model laboratory, we have been creating innovative humanized mouse which are reconstituted human hematopoietic and immune cells by transferring human CD34+ HSCs or PBMCs to recapitulate various human diseases including allergic, infectious, and autoimmune diseases, and GVHD. These models can be used to analyze in vivo onset mechanisms of the diseases and to apply preclinical evaluation of the efficacy and safety of novel drugs. We can provide those powerful tools to facilitate drug discovery research in worldwide.

ヒト化モデル研究室
※References
  1. Exacerbation of pathogenic Th17-cell-mediated cutaneous graft-versus-host-disease in human IL-1β and IL-23 transgenic humanized mice.
    Ito R, Katano I, Otsuka I, Hanazawa A, Takahashi T, Kawai K, Yagoto M, Goto M, OguraT, Takahashi R, Ito M.
    Biochem Biophys Res Commun. 2019 516(2): 480-5
  2. A humanized mouse model to study asthmatic airway inflammation via the human IL-33/IL-13 axis.
    Ito R, Maruoka S, Soda K, Katano I, Kawai K, Yagoto M, Hanazawa A, Takahashi T, Ogura T, Goto M, Takahashi R, Toyoshima S, Okayama Y, Izuhara K, Gon Y, Hashimoto S, Ito M, Nunomura S.
    JCI Insight. 2018 Nov 2;3(21)
  3. A novel in vivo model for predicting myelotoxicity of chemotherapeutic agents using IL-3/GM-CSF transgenic humanized mice
    Ito R, Nagai D, Igo N, Okuda Y, Sekine K, Ichimura E, Katano I, Mizushima T, Goto M, Ohnishi Y, Ito M, Okamoto K
    Toxicology Letter 2017 Nov. 5; 281: 152-157
  4. A Novel Xenogeneic Graft-Versus-Host Disease Model for Investigating the Pathological Role of Human CD4+ or CD8+ T Cells Using Immunodeficient NOG Mice.
    Ito R, Katano I, Kawai K, Yagoto M, Takahashi T, Ka Y, Ogura T, Takahashi R, Ito M.
    Am J Transplant. 2017 May;17(5):1216-1228
  5. Establishment of a human allergy model using human IL-3/GM-CSF transgenic NOG mice.
    Ito R, Takahashi T, Katano I, Kawai K, Kamisako T, Ogura T, Ida-Tanaka M, Suemizu H, Nunomura S, Ra C, Mori A, Aiso S, Ito M.
    Journal of Immunology 2013; 191:2890-2899
  6. Efficient Xenoengraftment in Severe Immunodeficient NOD/Shi-scid IL2rγnull Mice Is Attributed to a Lack of CD11c+B220+CD122+ cells
    Ito R, Katano I, Ida-Tanaka M, Kamisako T, Kawai K, Suemizu H, Aiso S, Ito M.
    Journal of Immunology 2012;189:4313-4320.
  7. Current advances in humanized mouse models
    Ito R, Takahashi T, Katano I, Ito M.
    Cellular and Molecular Immunology 2012;9:208-214.
  8. Highly sensitive model for xenogenic GVHD using severe immunodeficient NOG mice
    Ito R, Katano I, Kawai K, Hirata H, Ogura T, Kamisako T, Eto T, Ito M.
    Transplantation 2009;87:1654–1658.

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