Center of Basic Technology in Marmoset

Nowadays, there is an increased need for the Tg marmoset, a nonhuman primate, as a laboratory animal for the human disease model. We are a specialized team to perform the generation of genetically modified marmosets that enable approaching various diseases using genetic modification technology. Using the technology of generating genetically modified marmosets that originated in Japan, we are supporting research in medical science, drug discovery, and basic studies.

In the generation of genetically modified marmosets, comprensive tequniques of development of reproductive engineering and developmental engineering technologies, gestation monitoring, delivery, neonatal care, and rearing is necessary. Based on the principle of the 3Rs, our techniques are developing to obtain genetically modified marmosets by minimally invasive and more efficient.

We comprehensively manage the full range of techniques, including the gestation, delivery, neonatal care, and rearing of marmosets to generation of genetically modified marmosets. In addition to consigned generation of genetically modified marmosets, support for the following researches related to reproductive engineering and developmental engineering are being offered. Please feel free to contact us.

【Activities】

  1. Estrous cycle control for marmosets
    Rearing, pairing, blood collection, hormone measurements, and prediction of the time of ovulation
  2. Collection of marmoset oocytes
    Ovarian stimulation, hormone administration, surgeries for ovum-pic-up, postoperative management
  3. Developmental engineering technology
    In vitro maturation, sperm collection, in vitro fertilization, embryo culture, embryo transplantation
  4. Gestation monitoring and assisted delivery
    Hormone measurements, fetal diagnosis (confirmation of gestational sac, fetal heart beat, and measurement of fetal head circumference), determination of the expected date of delivery, monitoring during delivery, caesarean section
  5. Neonatal care
    Breast feeding care, feeding support, disease care
  6. Collection of preimplantation embryos (non-invasive preimplantation embryo collection collection)
    Cryopreservation and thawing of preimplantation embryos (from 8-cell to blastocyst stage)
Contact

Tg marmoset embryos ‐ exogenous gene introduction method using lentivirus vector

Inject virus fluid into the perivitelline space
Transplant normally developed embryos with the reporter protein (fluorescent) confirmed - Control (nonfluorescent) embryos in the middle.

Genome-edited marmoset embryos ‐ knockout technique using genome editing

Embryos with efficient genetic modification and low mosaic rate can be expected to be genetically modified individuals.

→Cel-1 assay conducted for mosaic rate test of embryos

eHiFi-ZFN 100% (8/8)
Platinum TALENs 100% (8/8)

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