Department of Applied Research for Laboratory Animals

Tumor Biology Laboratory

Tumor Biology Laboratory has established human tumor cell (xenograft) lines for over 30 years since the appearance of the nude mouse and has already established more than 500 xenograft lines. These xenografts have been provided to many researches for in vivo screening of anticancer drugs. Most recently, recipient mice have been changed from nude mice to NOG mice and it is now possible to produce xenograft models by transplantation of fewer cancer cells. Furthermore, liver metastasis models that are difficult to produce with nude mice or SCID (severe combined immunodeficiency) mice can be easily developed with good reproducibility using NOG mice. They can be used as models for drug efficacy evaluation. In addition, we revealed that the expression of the discovered factor in this NOG metastasis model is correlated with prognosis of liver metastasis by the analysis of clinical specimens.

Xenpgraft model with BALB/cA-nu/nu mice
  1. Whole-exome sequencing of 79 xenografts as a potential approach for the identification of genetic variants associated with sensitivity to cytotoxic anticancer drugs.
    Udagawa C, Sasaki Y, Tanizawa Y, Suemizu H, Ohnishi Y, Nakamura Y, Tokino T, Zembutsu H.
    PLoS One. 2020 Sep 28;15(9):e0239614.
  2. Fatty Acid β-Oxidation-dependent and -independent Responses and Tumor Aggressiveness Acquired Under Mild Hypoxia.
    Wakui M, Kawai K, Mizushima T, Nishime C, Serizawa A, Suemizu H, Asakura K, Yamauchi Y, Hayashida T, Suematsu M, Murata M.
    Anticancer Res. 2019 Jan;39(1):191-200.
  3. Establishment of patient-derived cancer xenografts in immunodeficient NOG mice.
    Chijiwa T, Kawai K, Noguchi A, Sato H, Hayashi A, Cho H, Shiozawa M, Kishida T, Morinaga S, Yokose T, Katayama M, Takenaka N, Suemizu H, Yamada R, Nakamura Y, Ohtsu T, Takano Y, Imai K, Miyagi Y, Nakamura M.
    International Journal of Oncology. 2015 Jul;47(1): 61-70.
  4. Zinc finger protein 185 is a liver metastasis-associated factor in colon cancer patients.
    Furukawa D, Chijiwa T, Matsuyama M, Mukai M, Matsuo EI, Nishimura O, Kawai K, Suemizu H, Hiraoka N, Nakagohri T, Yasuda S, Nakamura M.
    Mol Clin Oncol. 2014 Sep;2(5):709-713.
  5. A versatile technique for the in vivo imaging of human tumor xenografts using near-infrared fluorochrome-conjugated macromolecule probes.
    Suemizu H, Kawai K, Higuchi Y, Hashimoto H, Ogura T, Itoh T, Sasaki E, Nakamura M.
    PLoS One. 2013 Dec 17; 8(12): e82708.
  6. A new in vivo model to analyze hepatic metastasis of the human colon cancer cell line HCT116 in NOD/Shi-scid/IL-2Rγ(null) (NOG) mice by (18)F-FDG PET/CT.
    Kawai K, Tamura K, Sakata I, Ishida J, Nagata M, Tsukada H, Suemizu H, Nakamura M, Abe Y, Chijiwa T.
    Oncol Rep. 2013 Feb;29(2):464-8.


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